Cinchonin High quality/Best price/In stock CAS NO.118-10-5

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Cinchonin
118-10-5
standard supplier in China

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ProName: Cinchonin
CasNo: 118-10-5
Molecular Formula: C19H22N2O
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Cinchonine

Cas No.	118-10-5	SDF
PubChem ID	90454	Appearance	White powder
Formula	C₁₉H₂₂N₂O	M.Wt	294.39
Type of Compound	Alkaloids	Storage	Desiccate at -20°C
Solubility	DMSO : 4.76 mg/mL (16.17 mM; Need ultrasonic)
Chemical Name	(S)-[(2R,4S,5R)-5-ethenyl-1-azabicyclo[2.2.2]octan-2-yl]-quinolin-4-ylmethanol
SMILES	C=CC1CN2CCC1CC2C(C3=CC=NC4=CC=CC=C34)O
Standard InChIKey	KMPWYEUPVWOPIM-QAMTZSDWSA-N
Standard InChI	InChI=1S/C19H22N2O/c1-2-13-12-21-10-8-14(13)11-18(21)19(22)16-7-9-20-17-6-4-3-5-15(16)17/h2-7,9,13-14,18-19,22H,1,8,10-12H2/t13-,14-,18+,19-/m0/s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Cinchonine

The barks of Cinchona ledgeriana

Biological Activity of Cinchonine

Description	Cinchonine has antiplatelet effect, mediated mainly through inhibition of Ca2+-influx and protein kinase C pathways in platelets.Dietary Cinchonine with its effects on adipogenesis and inflammation may have a potential benefit in preventing obesity. Cinchonine may be used as an anti-multidrug resistance agent.
Targets	TLR \| Calcium Channel \| PKC \| PARP
In vitro	The inhibitory effect of cinchonine on human platelet aggregation due to blockade of calcium influx.[Pubmed: 9776305] Biochem Pharmacol. 1998 Oct 15;56(8):955-60. The Cinchona bark contains alkaloids like quinine, quinidine, Cinchonine and cinchonidine. These agents are effective antimalarial drugs and have been used clinically in malaria caused by Plasmodium falciparum. Previous studies show that quinine and quinidine exert effects on cardiovascular system. METHODS AND RESULTS: This study was conducted to examine the effect of Cinchonine on human platelet aggregation. The results show that Cinchonine inhibited platelet aggregation mediated by platelet agonists, epinephrine (200 microM), ADP (4.3 microM), platelet activating factor (PAF; 800 nM) and collagen (638 nM) but had no effect on arachidonic acid (AA; 0.75 mM). Cinchonine was most effective in inhibiting aggregation induced by platelet activating factor and epinephrine with IC50 values of 125 and 180 microM respectively, however, higher concentrations of Cinchonine were required to inhibit aggregation mediated by ADP or collagen (IC50; 300 microM). Pretreatment of platelets with Cinchonine inhibited aggregation caused by Ca2+ ionophore, A-23187 (6 microM), in a dose-dependent manner (IC50; 300 microM) indicating an inhibitory effect on Ca2+-signaling cascade. This was supported by measuring [Ca2+]i in platelets loaded with Fura-2AM where Cinchonine inhibited the rise in cytosolic Ca2+ mediated by A-23187 (6 microM) or collagen (638 nM). Results show that Cinchonine (20 microM) also inhibited aggregation when platelets were pretreated with protein kinase C (PKC) activator, phorbol myristate acetate (PMA; 0.1 microM) in combination with low doses of platelet activating factor (80 nM). Cinchonine, however, had no effect on AA-induced platelet aggregation and thromboxane A2 (TXA2) synthesis in platelets. CONCLUSIONS: These results suggest that antiplatelet effects of Cinchonine are mediated mainly through inhibition of Ca2+-influx and protein kinase C pathways in platelets.
In vivo	Cinchonine Prevents High-Fat-Diet-Induced Obesity through Downregulation of Adipogenesis and Adipose Inflammation.[Pubmed: 22675336] PPAR Res. 2012;2012:541204. Cinchonine (C(19)H(22)N(2)O) is a natural compound of Cinchona bark. Although Cinchonine's antiplatelet effect has been reported in the previous study, antiobesity effect of Cinchonine has never been studied. METHODS AND RESULTS: The main objective of this study was to investigate whether Cinchonine reduces high-fat-diet- (HFD-) induced adipogenesis and inflammation in the epididymal fat tissues of mice and to explore the underlying mechanisms involved in these reductions. HFD-fed mice treated with 0.05% dietary Cinchonine for 10 weeks had reduced body weight gain (-38%), visceral fat-pad weights (-26%), and plasma levels of triglyceride, free fatty acids, total cholesterol, and glucose compared with mice fed with the HFD. Moreover, Cinchonine significantly reversed HFD-induced downregulations of WNT10b and galanin-mediated signaling molecules and key adipogenic genes in the epididymal adipose tissues of mice. Cinchonine also attenuated the HFD-induced upregulation of proinflammatory cytokines by inhibiting toll-like-receptor-2- (TLR2-) and TLR4-mediated signaling cascades in the adipose tissue of mice. CONCLUSIONS: Our findings suggest that dietary Cinchonine with its effects on adipogenesis and inflammation may have a potential benefit in preventing obesity. Cinchonine, a potent efflux inhibitor to circumvent anthracycline resistance in vivo.[Pubmed: 1581892] Cancer Res. 1992 May 15;52(10):2797-801. Circumvention of multidrug resistance is a new field of investigation in cancer chemotherapy, and safe and potent multidrug resistance inhibitors are needed for clinical use. We investigated several analogues of quinine for their ability to increase anthracycline uptake in resistant cancer cells. Cinchonine was the most potent inhibitor of anthracycline resistance in vitro, and its activity was little altered by serum proteins. METHODS AND RESULTS: Serum from rats treated with i.v. Cinchonine produced greater uptake of doxorubicin in cancer cells (DHD/K12/PROb rat colon cells and K562/ADM human leukemic cells) than did serum from quinine-treated rats (ex vivo assay). Cinchonine was more effective than quinine in reducing tumor mass and increasing the survival of rats inoculated i.p. with DHD/K12/PROb cells and treated i.p. with deoxydoxorubicin. Moreover, the acute toxicity of Cinchonine in rats and mice was lower than that of other quinine-related compounds. CONCLUSIONS: The lower toxicity and greater potentiation of in vivo anthracycline activity produced by Cinchonine are favorable characteristics for its use as an anti-multidrug resistance agent in future clinical trials.

Protocol of Cinchonine

Cell Research

Hydrocinchonine, cinchonine, and quinidine potentiate paclitaxel-induced cytotoxicity and apoptosis via multidrug resistance reversal in MES-SA/DX5 uterine sarcoma cells.[Pubmed: 20196146]

Environ Toxicol. 2011 Aug;26(4):424-31.

Multidrug resistance (MDR) is one of important issues to cause the chemotherapy failure against cancers including gynecological malignancies. Despite some MDR reversal evidences of natural compounds including quinidine and Cinchonine, there are no reports on MDR reversal activity of hydroCinchonine with its analogues quinidine and Cinchonine especially in uterine sarcoma cells.
METHODS AND RESULTS:
Thus, in the current study, we comparatively investigated the potent efficacy of hydroCinchonine and its analogues quinidine and Cinchonine as MDR-reversal agents for combined therapy with antitumor agent paclitaxel (TAX). HydroCinchonine, Cinchonine, and quinidine significantly increased the cytotoxicity of TAX in P-glycoprotein (gp)-positive MES-SA/DX5, but not in the P-gp-negative MES-SA cells at nontoxic concentrations by 3-(4,5-dimethylthiazol-2-yl)-2,5--diphenyltetrazolium bromide (MTT) assay. Rhodamine assay also revealed that hydroCinchonine, Cinchonine, and quinidine effectively enhanced the accumulation of a P-gp substrate, rhodamine in TAX-treated MES-SA/DX5 cells compared with TAX-treated control. In addition, hydroCinchonine, Cinchonine, and quinidine effectively cleaved poly (ADP-ribose) polymerase (PARP), activated caspase-3, and downregulated P-gp expression as well as increased sub-G1 apoptotic portion in TAX-treated MES-SA/DX5 cells.
CONCLUSIONS:
Taken together, hydroCinchonine exerted MDR reversal activity and synergistic apoptotic effect with TAX in MES-SA/DX5 cells almost comparable with quinidine and Cinchonine as a potent MDR-reversal and combined therapy agent with TAX.

Preparing Stock Solutions of Cinchonine

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	3.3969 mL	16.9843 mL	33.9685 mL	67.9371 mL	84.9214 mL
5 mM	0.6794 mL	3.3969 mL	6.7937 mL	13.5874 mL	16.9843 mL
10 mM	0.3397 mL	1.6984 mL	3.3969 mL	6.7937 mL	8.4921 mL
50 mM	0.0679 mL	0.3397 mL	0.6794 mL	1.3587 mL	1.6984 mL
100 mM	0.034 mL	0.1698 mL	0.3397 mL	0.6794 mL	0.8492 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.