Peimisine CAS NO.19773-24-1

Min.Order: 10 Milligram
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Product Details

Keywords

Peimisine
19773-24-1
standard supplier in China

Quick Details

ProName: Peimisine
CasNo: 19773-24-1
Molecular Formula: C27H41NO3
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Peimisine

Cas No.	19773-24-1	SDF
PubChem ID	161294	Appearance	Powder
Formula	C₂₇H₄₁NO₃	M.Wt	427.62
Type of Compound	Alkaloids	Storage	Desiccate at -20°C
Synonyms	Ebeiensine
Solubility	Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name	(3S,3'R,3'aS,4aS,6'S,6aR,6bS,7'aR,9R,11aS,11bR)-3-hydroxy-3',6',10,11b-tetramethylspiro[1,2,3,4,4a,6,6a,6b,7,8,11,11a-dodecahydrobenzo[a]fluorene-9,2'-3a,4,5,6,7,7a-hexahydro-3H-furo[3,2-b]pyridine]-5-one
SMILES	CC1CC2C(C(C3(O2)CCC4C5CC(=O)C6CC(CCC6(C5CC4=C3C)C)O)C)NC1
Standard InChIKey	KYELXPJVGNZIGC-GKFGJCLESA-N
Standard InChI	InChI=1S/C27H41NO3/c1-14-9-24-25(28-13-14)16(3)27(31-24)8-6-18-19(15(27)2)11-21-20(18)12-23(30)22-10-17(29)5-7-26(21,22)4/h14,16-18,20-22,24-25,28-29H,5-13H2,1-4H3/t14-,16+,17-,18+,20-,21-,22+,24+,25-,26+,27-/m0/s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Peimisine

The bulbs of Fritillaria cirrhosa

Biological Activity of Peimisine

Description	Peimisine may have antihypertensive action, it can inhibit angiotensin I converting enzyme activity in a dose-dependent manner(the IC50 value of 526.5 microM). Peimisine can affect M-receptor, excit β-receptor, restrain the release of internal calcium, and promote to releaseing nitrogen monoxidum in order to relax tracheal smooth muscle and relieve asthma. It plays a protective role against LPS-induced acute lung injury, and against the experimental hepatic fibrosis formation.
Targets	NO \| SOD \| Calcium Channel
In vitro	Antiasthmatic mechanism of peimisine in Fritillaria monantha.[Reference: WebLink] Chinese Traditional & Herbal Drugs, 2009, 40(4):597-601. To approach the antiasthmatic mechanism of Peimisine in Fritillaria monatha. METHODS AND RESULTS: To observe the effect of Peimisine in the different concentration on the tracheal smooth muscle contraction in vitro organ induced by Ach, His, β-receptor, CaCl 2, Pro, in nitricoxide synthase catastaltica. Peimisine (0.046 and 0.092 mmol/L) made the EC 50 induced by Ach increased; The EC 50 of peimsine (0.092 mmol/L) was not changed by His; The EC 50 of Peimisine (0.092 mmol/L) was not significantly different to tracheal smooth muscle contraction induced by CaCl 2; Three dosages of Peimisine had significant inhibition on the release of intracellular calcium induced by CaCl 2 (P < 0.05, 0.01, and 0.001) in a dose-dependent manner. But extracellular calcium influx was not significantly inhibited. Comparing with the L-NAME+dissolvant group, three dosages of Peimisine couldn't restrain the contraction and had no significant difference. CONCLUSIONS: Peimisine could affect M-receptor, excit β-receptor, restrain the release of internal calcium, and promote to releaseing nitrogen monoxidum in order to relax tracheal smooth muscle and relieve asthma.
In vivo	Peimisine attenuates acute lung injury induced by lipopolysaccharide in mice[Reference: WebLink] Lishizhen Medicine & Materia Medica Research, 2014, 8(13-14):1842-7. To determine the protective effect of Peimisine on acute lung injury( ALI) induced by lipopolysaccharide( LPS) and its protective mechanism mice. METHODS AND RESULTS: The mice were randomly allocated into sham,LPS and Peimisine + LPS groups. The ALI mice was induced by LPS after etherization. The Peimisine was injected by belly cavity 30 minutes prior to the LPS challenge,one time/d,3 times totally. The mice were killed 24h after the third Peimisine injection to observe the amount of LDH,MDA in plasma,the lung tissue pathology,the total protein,white blood cell and differential count in the bronchoalveolar lavage fluid( BALF). LDH,MDA amount in plasma,Lung tissue,and BALF showed serious inflammatory changes in the LPS group. Compared with the LPS group,Peimisine attenuates Lung tissue injury,LDH and MDA amount in ALI mice in a dose dependent manner. Peimisine( 0. 12mg) lowered the total protein,total white blood cells,lymphocyte and neutrophilic leukocyte in BALF compared with the LPS group. CONCLUSIONS: Peimisine can play a protective role against LPS-induced acute lung injury.

Protocol of Peimisine

Animal Research

Protection of peimisine on hepatic fibrosis of rats induced by CCl4.[Reference: WebLink]

Chinese Traditional & Herbal Drugs, 2013, 44(11):1455-9.

To investigate the protective effect of Peimisine on carbon tetrachloride(CCl4)-induced hepatic fibrosis in rats.
METHODS AND RESULTS:
The rats were divided into control,model,low-,mid-,and high-dose(2.5,5,and 10 mg/kg) Peimisine groups.Hepatic fibrosis models were induced by ip injection of CCl4 in rats once every 3 d for 8 weeks.The rats in the treatment groups were administered four weeks after the model establishment,once daily until the end of the week 4 after the model establishment.The levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP),glutamyl transpeptidasecc(GGT),hyaluronie acid(HA),laminin(LN),type III procollagenc(PC-III),and collegen type IV(IV-C) were assayed,and hepatic tissue contents of hydroxyprolinc(Hyp),superoxide dismutase(SOD),and malondialdehyde(MDA) were determined.The effect of Peimisine on hepatic fibrosis in rats was observed. Compared with the model control group,the hepatic fibrosis of rats in Peimisine groups was improved obviously,the levels of ALT,AST,ALP,and GGT in serum were lowered obviously(P 0.05,0.01),also the serum levels of HA,LN,PC-III,IV-C,and the contents of Hyp and MDA in liver tissue were decreased(P 0.01),while the level of SOD was increased(P 0.01).
CONCLUSIONS:
Peimisine has the protective effect on the experimental hepatic fibrosis formation.The possible mechanisms are associated with inhibiting fibrogenesis and fibrosis accumulation,and decreasing lipid peroxidation.

Structure Identification

Planta Med. 2003 Jun;69(6):564-5.

Angiotensin converting enzyme (ACE) inhibitory alkaloids from Fritillaria ussuriensis.[Pubmed: 12865981 ]

Bioassay-guided fractionation of the BuOH-soluble extract of Fritillaria ussuriensis afforded verticinone ( 1), verticine ( 2), and Peimisine ( 3). Purification of these compounds was achieved with the use of various chromatographic methods.
METHODS AND RESULTS:
The structures of the compounds were identified on the basis of MS and NMR data analysis. Compounds 1 - 3 inhibited angiotensin I converting enzyme activity in a dose-dependent manner, displaying 50 % inhibitory concentration values of 165.0 microM, 312.8 microM, 526.5 microM, respectively.
CONCLUSIONS:
The presence of these active substances may be responsible, at least in part, for the antihypertensive action of the bulbs of Fritillaria ussuriensis.

Preparing Stock Solutions of Peimisine

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.3385 mL	11.6926 mL	23.3852 mL	46.7705 mL	58.4631 mL
5 mM	0.4677 mL	2.3385 mL	4.677 mL	9.3541 mL	11.6926 mL
10 mM	0.2339 mL	1.1693 mL	2.3385 mL	4.677 mL	5.8463 mL
50 mM	0.0468 mL	0.2339 mL	0.4677 mL	0.9354 mL	1.1693 mL
100 mM	0.0234 mL	0.1169 mL	0.2339 mL	0.4677 mL	0.5846 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.