Diosbulbin B CAS NO.20086-06-0

Min.Order: 10 Milligram
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Diosbulbin B
20086-06-0
standard supplier in China

Quick Details

ProName: Diosbulbin B
CasNo: 20086-06-0
Molecular Formula: C19H20O6
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Diosbulbin B

Cas No.	20086-06-0	SDF
PubChem ID	177107	Appearance	Powder
Formula	C₁₉H₂₀O₆	M.Wt	344.4
Type of Compound	Diterpenoids	Storage	Desiccate at -20°C
Solubility	DMSO : 16 mg/mL (46.46 mM; Need ultrasonic)
SMILES	CC12CC(OC13CC(C4C2CC5CC4C(=O)O5)OC3=O)C6=COC=C6
Standard InChIKey	QEANLIISUSNNDX-XBHMPIGQSA-N
Standard InChI	InChI=1S/C19H20O6/c1-18-6-13(9-2-3-22-8-9)25-19(18)7-14(24-17(19)21)15-11-4-10(5-12(15)18)23-16(11)20/h2-3,8,10-15H,4-7H2,1H3/t10-,11+,12+,13+,14-,15+,18-,19?/m0/s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Diosbulbin B

The roots of Dioscorea bulbifera

Biological Activity of Diosbulbin B

Description

Diosbulbin B has potential anti-tumor effects which may be related to influencing the immune system for the first time, it also exhibits potential hepatotoxicity.

Targets

P450 (e.g. CYP17)

In vivo

Ferulic acid prevents liver injury and increases the anti-tumor effect of diosbulbin B in vivo.[Pubmed: 24903991]

J Zhejiang Univ Sci B. 2014 Jun;15(6):540-7.

METHODS AND RESULTS:
The present study is designed to investigate the protection by ferulic acid against the hepatotoxicity induced by Diosbulbin B and its possible mechanism, and further observe whether ferulic acid augments Diosbulbin B-induced anti-tumor activity. The results show that ferulic acid decreases Diosbulbin B-increased serum alanine transaminase/aspartate transaminase (ALT/AST) levels. Ferulic acid also decreases lipid peroxide (LPO) levels which are elevated in Diosbulbin B-treated mice. Histological evaluation of the liver demonstrates hydropic degeneration in Diosbulbin B-treated mice, while ferulic acid reverses this injury. Moreover, the activities of copper- and zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) are decreased in the livers of Diosbulbin B-treated mice, while ferulic acid reverses these decreases. Further results demonstrate that the mRNA expressions of CuZn-SOD and CAT in Diosbulbin B-treated mouse liver are significantly decreased, while ferulic acid prevents this decrease. In addition, ferulic acid also augments Diosbulbin B-induced tumor growth inhibition compared with Diosbulbin B alone.
CONCLUSIONS:
Taken together, the present study shows that ferulic acid prevents Diosbulbin B-induced liver injury via ameliorating Diosbulbin B-induced liver oxidative stress injury and augments Diosbulbin B-induced anti-tumor activity.

Diosbulbin B-induced liver injury in mice and its mechanism.[Pubmed: 24107456]

Hum Exp Toxicol. 2014 Jul;33(7):729-36.

Dioscorea bulbifera L., a commonly used medicinal plant in China, is reported to induce hepatotoxicity. The present study is undertaken to investigate the hepatotoxicity induced by Diosbulbin B (DB), a diterpene lactone isolated from D. bulbifera L., and to further explore its underlying mechanism.
METHODS AND RESULTS:
DB was administered to mice at the doses of 0, 16, 32, and 64 mg/kg once daily for 12 consecutive days. Liver injury induced by Diosbulbin B was evidenced by the increased activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). Liver histological evaluation showed that the mice treated with Diosbulbin B exhibited liver damage with the swelling of hepatocytes. Further results showed that the amount of malondialdehyde (MDA) in the liver was increased in mice treated with Diosbulbin B, while the glutathione amount and the enzymatic activity of glutathione peroxidase (GPx), glutathione-S-transferase (GST), copper/zinc-superoxide dismutase (CuZn-SOD), manganese-SOD (Mn-SOD), and catalase (CAT) were all decreased. Diosbulbin B also decreased the gene expression of CuZn-SOD and CAT.
CONCLUSIONS:
Taken together, our results indicate that oral administration of Diosbulbin B for 12 consecutive days can lead to the oxidative stress liver injury in mice.

Protocol of Diosbulbin B

Kinase Assay

Cytochrome p450-mediated metabolic activation of diosbulbin B.[Pubmed: 25024403]

Metabolism of diosbulbin B in vitro and in vivo in rats: formation of reactive metabolites and human enzymes involved.[Pubmed: 25053620]

Drug Metab Dispos. 2014 Oct;42(10):1737-50.

Diosbulbin B (DB), a major constituent of the furano-norditerpenes in Dioscorea bulbifera Linn, exhibits potential antineoplasmic activity and hepatotoxicity. The metabolism and reactive metabolites of Diosbulbin B in vitro (with human and animal liver microsomes) and in vivo in rats were investigated.
METHODS AND RESULTS:
The human enzymes involved in DB metabolism were identified. Diosbulbin B was first catalyzed into reactive metabolites of 2-butene-1,4-dial derivatives dependent on NADPH and then trapped by Tris base or oxidized to hemiacetal lactones (M12 and M13) in microsomal incubations. Tris base was used as buffer constituent and as a trapping agent for aldehyde. Methoxylamine and glutathione (GSH) were also used as trapping agents. Diosbulbin B metabolism in vivo in rats after oral administration was consistent with that in vitro. The structures of M12 and M13, as well as mono-GSH conjugates of Diosbulbin B (M31), were confirmed by nuclear magnetic resonance spectroscopy of the chemically synthesized products. The bioactivation enzymes of Diosbulbin B were identified as CYP3A4/5, 2C9, and 2C19. CYP3A4 was found to be the primary enzyme using human recombinant cytochrome P450 enzymes, specific inhibitory studies, and a relative activity factor approach for pooled human liver microsomes. Michaelis-Menten constants K(m) and V(max) were determined by the formation of M31.
CONCLUSIONS:
The reactive metabolites may be related to the hepatotoxicity of Diosbulbin B. The gender difference in CYP3A expression in mice and rats contributed to the gender-related liver injury and pharmacokinetics in mice and rats, respectively.

Drug Metab Dispos. 2014 Oct;42(10):1727-36.

Diosbulbin B (DIOB), a furan-containing diterpenoid lactone, is the most abundant component of Dioscorea bulbifera L. (DB), a traditional Chinese medicine herb. Administration of purified Diosbulbin B or DB extracts has been reported to cause liver injury in animals. The mechanisms of Diosbulbin B-induced hepatotoxicity remain unknown. The major objective of this study was to identify reactive metabolites of Diosbulbin B.
METHODS AND RESULTS:
A Diosbulbin B-derived cis-enedial was trapped by N-acetyl lysine (NAL) and glutathione (GSH) or N-acetyl cysteine (NAC) in rat and human liver microsomal incubation systems after exposure to Diosbulbin B. Four metabolites (M1-M4) associated with GSH were detected by liquid chromatography coupled to tandem mass spectrometry. Apparently, M1 was derived from both NAL and GSH. M2 and M3 resulted from the reaction of GSH without the involvement of NAL. Two molecules of GSH participated in the formation of M4. M2 and M3 were also detected in bile and urine of rats given Diosbulbin B. M5, a Diosbulbin B-derived NAC/NAL conjugate, was detected in microsomal incubations with Diosbulbin B fortified with NAC and NAL as trapping agents. Biomimetic M1-M5 were prepared by oxidation of Diosbulbin B with Oxone for metabolite identification.
CONCLUSIONS:
Microsomal incubation study demonstrated that ketoconazole inhibited the production of the enedial in a concentration-dependent manner, and CYP3A4 was found to be the enzyme responsible for the metabolic activation of Diosbulbin B. The metabolism study facilitates the understanding of the role of bioactivation of Diosbulbin B in its hepatotoxicity.

Preparing Stock Solutions of Diosbulbin B

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.9036 mL	14.518 mL	29.036 mL	58.072 mL	72.59 mL
5 mM	0.5807 mL	2.9036 mL	5.8072 mL	11.6144 mL	14.518 mL
10 mM	0.2904 mL	1.4518 mL	2.9036 mL	5.8072 mL	7.259 mL
50 mM	0.0581 mL	0.2904 mL	0.5807 mL	1.1614 mL	1.4518 mL
100 mM	0.029 mL	0.1452 mL	0.2904 mL	0.5807 mL	0.7259 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.