Bardoxolone Methyl High quality with Factory price CAS NO.218600-53-4

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers. 
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1.Provide traditional Chinese medicine reference materials and natural active ingredients;
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General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Chemical Properties of Bardoxolone methyl

Biological Activity of Bardoxolone methyl

Protocol

Kinase Assay [1]
Kidney tissue from the 28-day monkey study is snap-frozen in liquid nitrogen, and stored at -80°C until analysis. Kidney tissue is homogenized at 250 mg tissue/mL ice cold PBS (pH 7.2), containing 2 mM ethylenediaminetetraacetic acid (EDTA). Homogenates are then centrifuged at 10,000 x g for 10 minutes at 4°C. The supernatants are collected and stored at -80°C until analysis. Protein concentrations of tissue homogenates are determined using the Bicinchoninic Acid (BCA) Protein Assay Kit from Pierce Biotechnology. NQO1 enzyme activity is determined by quantifying the rate of reduction of 2,6-dichlorophenol-indophenol (DCPIP). Gsr enzyme activity is determined using a commercially available kit. All enzyme activities are normalized to protein and presented as fold vehicle control.

Animal Administration [1]
Two separate in-life studies are conducted in cynomolgus monkeys. In one study, cynomolgus monkeys (n=9/gender/dose group) are administered amorphous bardoxolone methyl by oral gavage, using sesame oil as the vehicle, at 5, 30, and 300 mg/kg once daily for 12 months in a GLP environment. Observations for morbidity, mortality, injury, and the availability of food and water are conducted twice daily for all animals. Clinical observations and body weights are conducted and recorded weekly. Weight data are analyzed by calculating the area under the weight versus time curve using the linear trapezoidal method. Blood samples for clinical chemistry evaluations are collected from all animals pretest and from all animals prior to interim (6-month) and terminal (12-month) necropsies. An additional group of monkeys for each dose group are allowed to recover for 4 weeks.

References:
[1]. Reisman SA, et al. Bardoxolone Methyl Decreases Megalin and Activates Nrf2 in the Kidney. J Am Soc Nephrol. 2012 Aug 2. [2]. McCullough PA, et al. Cardiac and renal function in patients with type 2 diabetes who have chronic kidney disease: potential effects of bardoxolone methyl. Drug Des Devel Ther. 2012;6:141-9.

Preparing Stock Solutions of Bardoxolone methyl