Tryptophol High quality with Factory price CAS NO.526-55-6

Min.Order: 10 Milligram
Payment Terms: T/T
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Tryptophol
526-55-6
standard supplier in China

Quick Details

ProName: Tryptophol
CasNo: 526-55-6
Molecular Formula: C10H11NO
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Tryptophol

Cas No.	526-55-6	SDF
PubChem ID	10685	Appearance	Powder
Formula	C₁₀H₁₁NO	M.Wt	161.2
Type of Compound	Alkaloids	Storage	Desiccate at -20°C
Solubility	Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name	2-(1H-indol-3-yl)ethanol
SMILES	C1=CC=C2C(=C1)C(=CN2)CCO
Standard InChIKey	MBBOMCVGYCRMEA-UHFFFAOYSA-N
Standard InChI	InChI=1S/C10H11NO/c12-6-5-8-7-11-10-4-2-1-3-9(8)10/h1-4,7,11-12H,5-6H2
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Tryptophol

The Zygosaccharomyces priorianus.

Biological Activity of Tryptophol

Description	Tryptophol is an aromatic alcohol found as a secondary product of alcoholic fermentation that induces sleep in humans. Tryptophol can inhibit the infection/replication of GVE2 by interacting with the host's Clp protease. Tryptophol can inhibit the proliferation of U937 cells by inducing apoptosis via a pathway involving caspase-8 followed by caspase-3. Tryptophol induces apoptosis through Death Receptor (DR) 5 and that the resistance of peripheral blood lymphocytes (PBL) to tryptophol-induced apoptosis might be due to competition from decoy receptor (DcR).
Targets	PARP \| Caspase
In vitro	Tryptophol induces death receptor (DR) 5-mediated apoptosis in U937 cells.[Pubmed: 17690453] Biosci Biotechnol Biochem. 2007 Aug;71(8):2065-8. Tryptophol is a natural component isolated from vinegar produced from the boiled extract of black soybean. We have reported that Tryptophol induces apoptosis in U937 cells via activation of caspase-8 followed by caspase-3. Tryptophol, however, did not affect human peripheral blood lymphocytes (PBL). METHODS AND RESULTS: In this study, we found that Tryptophol enhances formation of a death-inducing signaling complex including death receptor (DR) 5. Cell viability and induction of apoptosis by Tryptophol was reduced by transfection with decoy receptor (DcR) 1. CONCLUSIONS: These results indicate that Tryptophol induces apoptosis through DR5 and that the resistance of PBL to Tryptophol-induced apoptosis might be due to competition from DcR1. Genotoxicity of tryptophol in a battery of short-term assays on human white blood cells in vitro.[Pubmed: 18248512] Basic Clin Pharmacol Toxicol. 2008 May;102(5):443-52. METHODS AND RESULTS: The genotoxic effects of Tryptophol (indole-3-ethanol), an aromatic alcohol and known secondary metabolite of the opportunistic yeast Candida albicans and other Candida spp. The results obtained suggest cytotoxic, cytostatic and genotoxic effects of the Tryptophol treatment in vitro that were mainly dose-dependent. The type and the extent of DNA lesions detected in Tryptophol-treated samples indicate the possibility that observed damage is mediated by highly reactive aldehyde metabolite and/or free radicals produced by treatment. The results show that mortality of lymphocytes in Tryptophol-treated samples was primarily caused by apoptosis. The generation of additional DNA strand breaks and cytogenetic consequences (chromosome aberrations, sister chromatid exchanges and micronuclei), as observed in this study, sustain the possibility that Tryptophol toxicity is mediated by the formation of DNA cross-links and aldehyde-protein adducts. CONCLUSIONS: In conclusion, this preliminary study elucidates only a part of Tryptophol toxicity to human cells. Because current evidence is not sufficient to obtain information relevant for human risk assessment, further in vitro and in vivo studies are essential in order to clarify remaining issues, especially to elucidate the exact mechanisms and nature of the damage produced following treatment as well to estimate possible interindividual variability in genotoxic responses to the chemical. The effects of tryptophol on immune responses and it implications toward trypanosome-induced immunosuppression.[Pubmed: 776647] Experientia. 1976 May 15;32(5):645-7. METHODS AND RESULTS: Daily administration of Tryptophol to laboratory rodents resulted in significantly depressed antibody production of heterologous red blood cell challenge, did not alter cellular-mediated responses to oxazalone. CONCLUSIONS: These results suggest that trypanosome-produced Tryptophol may account for the immunodepression observed during trypanosomiasis.
In vivo	Tryptophol, 5-hydroxytryptophol and 5-methoxytryptophol induced sleep in mice.[Reference: WebLink] Life Sci. 1970 Mar 15;9(6):323-9. METHODS AND RESULTS: Tryptophol, 5-hydroxyTryptophol and 5-methoxyTryptophol were found to induce sleep in mice. The onset of action was most rapid for Tryptophol and 5-methoxyTryptophol; 5-hydroxyTryptophol had the slowest onset, probably due to difficulty of transport across the blood-brain barrier. Sleep times were shortest for Tryptophol and 5-methoxyTryptophol and longest for 5-hydroxyTryptophol. CONCLUSIONS: It was suggested that the Tryptophols or the corresponding aldehydes may play a role in physiological sleep mechanisms.

Protocol of Tryptophol

Kinase Assay

Isolation of tryptophol as an apoptosis-inducing component of vinegar produced from boiled extract of black soybean in human monoblastic leukemia U937 cells.[Pubmed: 17284845]

Biosci Biotechnol Biochem. 2007 Feb;71(2):371-9.

We isolated a novel apoptosis-inducing component, Tryptophol, from vinegar produced from boiled extract of black soybean (black soybean vinegar).
METHODS AND RESULTS:
Compound-6 purified from an ethyl acetate extract of black soybean vinegar using high performance liquid chromatography (HPLC) induced fragmentation of DNA and the development of apoptotic bodies (characteristic physiological features of apoptosis) in U937 cells. By analysis of chemical structure, this active compound was identified as Tryptophol. Tryptophol induced apoptosis involving caspase-8 and -3 activation, followed by cleavage of poly (ADP-ribose) polymerase (PARP), as shown by measurement of enzyme activity and immunoblot analysis. The cell viability of normal lymphocytes separated from human blood was less affected by Tryptophol, and fragmentation of DNA was not induced in normal lymphocytes.
CONCLUSIONS:
These results indicate that Tryptophol isolated from black soybean vinegar inhibited the proliferation of U937 cells by inducing apoptosis via a pathway involving caspase-8 followed by caspase-3, without affecting normal lymphocytes.

Preparing Stock Solutions of Tryptophol

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	6.2035 mL	31.0174 mL	62.0347 mL	124.0695 mL	155.0868 mL
5 mM	1.2407 mL	6.2035 mL	12.4069 mL	24.8139 mL	31.0174 mL
10 mM	0.6203 mL	3.1017 mL	6.2035 mL	12.4069 mL	15.5087 mL
50 mM	0.1241 mL	0.6203 mL	1.2407 mL	2.4814 mL	3.1017 mL
100 mM	0.062 mL	0.3102 mL	0.6203 mL	1.2407 mL	1.5509 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.