Rebaudioside A CAS NO.58543-16-1

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General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
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Chemical Properties of Rebaudioside A

Source of Rebaudioside A

The leaves of Stevia rebaudiana Bertoni.

Biological Activity of Rebaudioside A

Protocol of Rebaudioside A

Preparing Stock Solutions of Rebaudioside A

References on Rebaudioside A

The non-cytotoxicity characterization of rebaudioside A as a food additive.[Pubmed:24500608]

Food Chem Toxicol. 2014 Apr;66:334-40.

To evaluate the cytotoxicity of high-purity Rebaudioside A (reb A, 99.16%) as a food ingredient, a combination of several methods, including tetrazolium-based colorimetric assay (MTT), lactate dehydrogenase assay (LDH), enzyme-linked immunosorbent assay (ELISA), real-time PCR (qPCR), high-performance liquid chromatography (HPLC), and two-dimensional electrophoresis (2-DE) were used to test the cytotoxicity of reb A on the human cells HT-29 and T84, as well as liver and spleen cells from mice. The results indicated that no obvious changes in cellular viability, inflammatory cytokines yield, or protein yield were observed between the test group and the control group when different concentrations of reb A were used, suggesting that reb A is non-cytotoxic in vitro at the concentrations range tested (0.001-0.5%).

NMR studies of the conformation of the natural sweetener rebaudioside A.[Pubmed:19889398]

Carbohydr Res. 2009 Dec 14;344(18):2533-8.

Rebaudioside A is a natural sweetener from Stevia rebaudiana in which four beta-D-glucopyranose units are attached to the aglycone steviol. Its (1)H and (13)C NMR spectra in pyridine-d(5) were assigned using 1D and 2D methods. Constrained molecular dynamics of solvated rebaudioside using NMR constraints derived from ROESY cross peaks yielded the orientation of the beta-D-glucopyranose units. Hydrogen bonding was examined using the temperature coefficients of the hydroxyl chemical shifts, ROESY and long-range COSY spectra, and proton-proton coupling constants.

Bioconversion of rebaudioside I from rebaudioside A.[Pubmed:25353385]

Molecules. 2014 Oct 28;19(11):17345-55.

To supply the increasing demand of natural high potency sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of Rebaudioside A to rebaudioside I using a glucosyltransferase enzyme. This bioconversion reaction adds one sugar unit with a 1-->3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.

Description

Rebaudioside A is a steviol glycoside, α-glucosidase inhibitor with IC50 of 35.01 μg/ml.

Keywords:

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