- Product Details
Keywords
- Rotenone
- 83-79-4
- standard supplier in China
Quick Details
- ProName: Rotenone
- CasNo: 83-79-4
- Molecular Formula: C23H22O6=394.42
- Appearance: detailed see specifications
- Application: analysis,activity test,Botanical Refer...
- DeliveryTime: 1-3?working?days?after?confirming?the?...
- PackAge: According to the clients requirement.
- Port: China main port
- ProductionCapacity: 1 Metric Ton/Day
- Purity: ≥98%
- Storage: Store at 2~8°C
- Transportation: by air or by ocean shipping
- LimitNum: 10 Milligram
- Plant of Origin: Chinese herbal medicine
- Testing Method: NMR/MS/HPLC
- Product Ecification: 1mg-1kg
- Heavy Metal: <10ppm
- Voluntary Standards: company standard
- Storage: Store in dry, dark and ventilated plac...
- PackAge: Brown vial HDPE plastic bottle
Superiority
Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine,Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.
Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library
1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging:1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition:Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.
Details
Chemical Properties of Rotenone
Cas No. | 83-79-4 | SDF | |
PubChem ID | 6758 | Appearance | Cryst. |
Formula | C23H22O6 | M.Wt | 394.42 |
Type of Compound | Miscellaneous | Storage | Desiccate at -20°C |
Solubility | DMSO : 50 mg/mL (126.77 mM; Need ultrasonic) H2O : < 0.1 mg/mL (insoluble) |
||
SMILES | CC(=C)C1CC2=C(O1)C=CC3=C2OC4COC5=CC(=C(C=C5C4C3=O)OC)OC | ||
Standard InChIKey | JUVIOZPCNVVQFO-HBGVWJBISA-N | ||
Standard InChI | InChI=1S/C23H22O6/c1-11(2)16-8-14-15(28-16)6-5-12-22(24)21-13-7-18(25-3)19(26-4)9-17(13)27-10-20(21)29-23(12)14/h5-7,9,16,20-21H,1,8,10H2,2-4H3/t16-,20-,21+/m1/s1 | ||
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. |
Source of Rotenone
The herbs of Derris trifoliata Lour.
Biological Activity of Rotenone
Description | Rotenone is a mitochondrial complex I inhibitor that produces an animal model of Parkinson's disease. Rotenone-induced α-synuclein aggregation is mediated by the calcium/GSK3β signaling pathway. Rotenone can increase intracellular levels of the toxic dopamine metabolite 3,4-dihydroxyphenyl-acetaldehyde (DOPAL), via decreasing DOPAL metabolism by aldehyde dehydrogenase (ALDH) and decreasing vesicular sequestration of cytoplasmic dopamine by the vesicular monoamine transporter (VMAT). |
Targets | Akt | GSK-3 | ROS | JNK | VMAT2 |
In vitro |
GAPDH-knockdown reduce rotenone-induced H9C2 cells death via autophagy and anti-oxidative stress pathway.[Pubmed: 25725130] Toxicol Lett. 2015 May 5;234(3):162-71. GAPDH, well known for its house-keeping functions, has also been shown to be involved in cell injury, apoptosis and death under conditions of stress such as starvation, chemical injury and oxidative stress. This study examines the effect of GAPDH knockdown on cell injury in response to Rotenone. JNK inhibition of VMAT2 contributes to rotenone-induced oxidative stress and dopamine neuron death.[Pubmed: 25496994] Toxicology. 2015 Feb 3;328:75-81. Treatment with Rotenone, both in vitro and in vivo, is widely used to model dopamine neuron death in Parkinson's disease upon exposure to environmental neurotoxicants and pesticides. Mechanisms underlying Rotenone neurotoxicity are still being defined. |
Protocol of Rotenone
Kinase Assay |
The molecular mechanism of rotenone-induced α-synuclein aggregation: emphasizing the role of the calcium/GSK3β pathway.[Pubmed: 25433145] Toxicol Lett. 2015 Mar 4;233(2):163-71. Environmental toxin exposure is associated with the development of Parkinson's disease (PD), and environmental factors can influence the onset of the majority of sporadic PD cases via genetically mediated pathways. Rotenone, a widespread pesticide, induces Parkinsonism and the formation of Lewy bodies in animals; however, the molecular mechanism that underlies α-synuclein aggregation remains unclear. |
Cell Research |
Rotenone decreases intracellular aldehyde dehydrogenase activity: implications for the pathogenesis of Parkinson's disease.[Pubmed: 25645689] J Neurochem. 2015 Apr;133(1):14-25. Repeated systemic administration of the mitochondrial complex I inhibitor Rotenone produces a rodent model of Parkinson's disease (PD). Mechanisms of relatively selective Rotenone-induced damage to nigrostriatal dopaminergic neurons remain incompletely understood. According to the 'catecholaldehyde hypothesis,' buildup of the autotoxic dopamine metabolite 3,4-dihydroxyphenylacetaldehyde (DOPAL) contributes to PD pathogenesis. Vesicular uptake blockade increases DOPAL levels, and DOPAL is detoxified mainly by aldehyde dehydrogenase (ALDH). |
Preparing Stock Solutions of Rotenone
1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
1 mM | 2.5354 mL | 12.6768 mL | 25.3537 mL | 50.7074 mL | 63.3842 mL |
5 mM | 0.5071 mL | 2.5354 mL | 5.0707 mL | 10.1415 mL | 12.6768 mL |
10 mM | 0.2535 mL | 1.2677 mL | 2.5354 mL | 5.0707 mL | 6.3384 mL |
50 mM | 0.0507 mL | 0.2535 mL | 0.5071 mL | 1.0141 mL | 1.2677 mL |
100 mM | 0.0254 mL | 0.1268 mL | 0.2535 mL | 0.5071 mL | 0.6338 mL |
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. |