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Limonin CAS NO.1180-71-8
- Min.Order: 10 Milligram
- Payment Terms: T/T
- Available Specifications:
- Product Details
Keywords
- Limonin
- 1180-71-8
- standard supplier in China
Quick Details
- ProName: Limonin
- CasNo: 1180-71-8
- Molecular Formula: C26H30O8=470.5
- Appearance: detailed see specifications
- Application: analysis,activity test,Botanical Refer...
- DeliveryTime: 1-3?working?days?after?confirming?the?...
- PackAge: According to the clients requirement.
- Port: China main port
- ProductionCapacity: 1 Metric Ton/Day
- Purity: ≥98%
- Storage: Store at 2~8°C
- Transportation: by air or by ocean shipping
- LimitNum: 10 Milligram
- Plant of Origin: Chinese herbal medicine
- Testing Method: NMR/MS/HPLC
- Product Ecification: 1mg-1kg
- Heavy Metal: <10ppm
- Voluntary Standards: company standard
- Storage: Store in dry, dark and ventilated plac...
- PackAge: Brown vial HDPE plastic bottle
Superiority
Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.
Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library
1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries
General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.
Details
Chemical Properties of Limonin
| Cas No. | 1180-71-8 | ||
| PubChem ID | 179651 | Appearance | White powder |
| Formula | C26H30O8 | M.Wt | 470.5 |
| Type of Compound | Triterpenoids | Storage | Desiccate at -20°C |
| Synonyms | Limonoic acid 3,19:16,17 dilactone | ||
| Solubility | DMSO : ≥ 110 mg/mL (233.79 mM) *"≥" means soluble, but saturation unknown. |
||
| SMILES | CC1(C2CC(=O)C3(C(C24COC(=O)CC4O1)CCC5(C36C(O6)C(=O)OC5C7=COC=C7)C)C)C | ||
| Standard InChIKey | KBDSLGBFQAGHBE-MSGMIQHVSA-N | ||
| Standard InChI | InChI=1S/C26H30O8/c1-22(2)15-9-16(27)24(4)14(25(15)12-31-18(28)10-17(25)33-22)5-7-23(3)19(13-6-8-30-11-13)32-21(29)20-26(23,24)34-20/h6,8,11,14-15,17,19-20H,5,7,9-10,12H2,1-4H3/t14-,15-,17-,19-,20+,23-,24-,25+,26+/m0/s1 | ||
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it. |
||
| About Packaging | 1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment. |
||
| Shipping Condition | Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request. | ||
Source of Limonin
1 Dictamnus sp.
Biological Activity of Limonin
| Description | Limonin is a widely used dietary supplement, one of the most prevalent citrus limonoids, which has antioxidant, anti-inflammatory, anticancer and anti-human immunodeficiency virus(HIV)activity. It induced a down regulation of TLR-2 ,TLR-4,TNF-α, TNF-α/IL-10,NF-κB and caspase. It showed the potent inhibition of CYP3A4, with IC50 values of 6.20 μM (CYP3A4/testosterone) and 19.10 μM (CYP3A4/midazolam). |
| Targets | HIV | P450 (e.g. CYP17) | TLR |
| In vitro |
Influence of limonin on Wnt signalling molecule in HepG2 cell lines.[Pubmed: 23633848] J Nat Sci Biol Med. 2013 Jan;4(1):126-33. The role of Limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies. Effect of limonin and nomilin on HIV-1 replication on infected human mononuclear cells.[Pubmed: 14648393] Planta Med. 2003 Oct;69(10):910-3. In the last years several plant-derived natural compounds have been screened for their anti-HIV activity in order to find lead compounds with novel structures or mechanisms of action. Among these, several triterpenoids have been found to exhibit an antiretroviral activity with different mechanisms of action. |
| In vivo |
Limonin attenuates hepatocellular injury following liver ischemia and reperfusion in rats via toll-like receptor dependent pathway.[Pubmed: 24967531] Eur J Pharmacol. 2014 Oct 5;740:676-82. Limonin has been shown to exhibit anti-inflammatory and antioxidant properties in the settings of chemically induced hepatic injury. |
Protocol of Limonin
| Kinase Assay |
Inhibitory effects of limonin on six human cytochrome P450 enzymes and P-glycoprotein in vitro.[Pubmed: 22001672] Toxicol In Vitro. 2011 Dec;25(8):1828-33. Among the various possible causes for drug interactions, pharmacokinetic factors such as inhibition of drug-metabolizing enzymes and transporters, especially cytochrome P450 (CYP) isoenzymes and P-glycoprotein (P-gp), are regarded as the most frequent and clinically important. Limonin is a widely used dietary supplement and one of the most prevalent citrus limonoids, which are known to have inhibitory effects on CYPs and P-gp. |
| Cell Research |
Effect of limonin and nomilin on HIV-1 replication on infected human mononuclear cells.[Pubmed: 14648393] Planta Med. 2003 Oct;69(10):910-3. Cell lines:PBMC, and M/M cells |
Preparing Stock Solutions of Limonin
| 1 mg | 5 mg | 10 mg | 20 mg | 25 mg | |
| 1 mM | 2.1254 mL | 10.627 mL | 21.254 mL | 42.508 mL | 53.135 mL |
| 5 mM | 0.4251 mL | 2.1254 mL | 4.2508 mL | 8.5016 mL | 10.627 mL |
| 10 mM | 0.2125 mL | 1.0627 mL | 2.1254 mL | 4.2508 mL | 5.3135 mL |
| 50 mM | 0.0425 mL | 0.2125 mL | 0.4251 mL | 0.8502 mL | 1.0627 mL |
| 100 mM | 0.0213 mL | 0.1063 mL | 0.2125 mL | 0.4251 mL | 0.5313 mL |
| * Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations. | |||||
References on Limonin
Limonin attenuates hepatocellular injury following liver ischemia and reperfusion in rats via toll-like receptor dependent pathway.[Pubmed:24967531]
Eur J Pharmacol. 2014 Oct 5;740:676-82.
Limonin has been shown to exhibit anti-inflammatory and antioxidant properties in the settings of chemically induced hepatic injury. The current study aimed to investigate the protective effects of Limonin on experimentally-induced hepatic ischemia reperfusion (I/R) injury in rats. Rats were injected IP with either DMSO or Limonin (100 mg/kg BW), 30 min before submission to 45 min of ischemia, followed by 1 h of reperfusion. Limonin ameliorated the deleterious effects of I/R as indicated by improvement in liver function tests, reduction of lactate dehydrogenase, reduction of oxidative stress, decrease in hepatocyte degeneration, and pyknosis. Furthermore, pretreatment of I/R rats with Limonin, induced a significant down regulation in the various elements of the toll like receptor (TLR)pathway including TLR-2 and TLR-4, myeloid differentiation factor 88 (MYD88) and toll/IR-1(TIR)-domain-containing adaptor protein inducing interferon-beta (TRIF) and the downstream effectors TNF-alpha, TNF-alpha/IL-10 ratio and nuclear factor-kappaB (NF-kappaB). It also increased the anti-inflammatory cytokine IL-10 and decreased the activity of the apoptotic marker, caspase-3. These data indicate that Limonin exerts antioxidant and anti-inflammatory effects in ischemic liver, thus, protecting hepatocytes against I/R injury in rats. The mechanism of these hepatoprotective effects appears to be related to the antioxidant and anti-inflammatory potential of Limonin mediated by the down regulation of TLR- signaling pathway.
Influence of limonin on Wnt signalling molecule in HepG2 cell lines.[Pubmed:23633848]
J Nat Sci Biol Med. 2013 Jan;4(1):126-33.
OBJECTIVE: The role of Limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies. MATERIALS AND METHODS: In this study, Limonin is identified as a potent anti proliferative agent against human hepatoma HepG2 cells based on the cell viability study, LDH leakage assay. Induction of apoptosis in HepG2 cells by Limonin was evidenced by western blot analysis of Bax, Cyclin D1, Caspase 3 and Caspase9. RESULTS: Since Wnt signalling is involved in the initiation and sustaining of hepatocellular carcinoma we studied differential expression of LRP5, LRP6 and DKK wnt players. CONCLUSION: Limonin found to down regulate these players which forms a rationale for further investigation on effect on Limonin in cancer therapy.
Inhibitory effects of limonin on six human cytochrome P450 enzymes and P-glycoprotein in vitro.[Pubmed:22001672]
Toxicol In Vitro. 2011 Dec;25(8):1828-33.
Among the various possible causes for drug interactions, pharmacokinetic factors such as inhibition of drug-metabolizing enzymes and transporters, especially cytochrome P450 (CYP) isoenzymes and P-glycoprotein (P-gp), are regarded as the most frequent and clinically important. Limonin is a widely used dietary supplement and one of the most prevalent citrus limonoids, which are known to have inhibitory effects on CYPs and P-gp. In this study, the in vitro inhibitory effects of Limonin on the major human CYP isoenzymes (CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and CYP3A4) activities in human liver microsomes were examined using liquid chromatography-tandem mass spectrometry. The inhibitory effects of Limonin on P-gp activity in a human metastatic malignant melanoma cell line WM-266-4 were examined using a calcein-AM fluorometry screening assay. It demonstrates that Limonin has negligible inhibitory effects on human CYP1A2, CYP2C8, CYP2C9, CYP2C19, CYP2D6, and P-gp. However, potent inhibition of CYP3A4 by Limonin is observed with IC50 values of 6.20 muM (CYP3A4/testosterone) and 19.10 muM (CYP3A4/midazolam). This finding has important implications with regard to food-drug interactions between Limonin and several narrow therapeutic index drugs that are metabolized by CYP3A4.
Description
Limonin is a triterpenoid enriched in citrus fruits, which has antivirus and antitumor ability.
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