Vincristine Sulfate CAS NO.2068-78-2

Min.Order: 10 Milligram
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Keywords

Vincristine Sulfate
2068-78-2
standard supplier in China

Quick Details

ProName: Vincristine Sulfate
CasNo: 2068-78-2
Molecular Formula: C46H56N4O10.H2SO4
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Vincristine sulfate

Cas No.	2068-78-2	SDF
PubChem ID	249332	Appearance	Powder
Formula	C₄₆H₅₈N₄O₁₄S	M.Wt	923.04
Type of Compound	Alkaloids	Storage	Desiccate at -20°C
Synonyms	Leurocristine sulfate; 22-Oxovincaleukoblastine sulfate
Solubility	DMSO : 82.5 mg/mL (89.38 mM; Need ultrasonic)
SMILES	CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C=O)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
Standard InChIKey	AQTQHPDCURKLKT-JKDPCDLQSA-N
Standard InChI	InChI=1S/C46H56N4O10.H2O4S/c1-7-42(55)22-28-23-45(40(53)58-5,36-30(14-18-48(24-28)25-42)29-12-9-10-13-33(29)47-36)32-20-31-34(21-35(32)57-4)50(26-51)38-44(31)16-19-49-17-11-15-43(8-2,37(44)49)39(60-27(3)52)46(38,56)41(54)59-6;1-5(2,3)4/h9-13,15,20-21,26,28,37-39,47,55-56H,7-8,14,16-19,22-25H2,1-6H3;(H2,1,2,3,4)/t28-,37+,38-,39-,42+,43-,44-,45+,46+;/m1./s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Vincristine sulfate

The herbs of Hedera nepalensis K. Koch var. sinensis (Tobl.) Rehd.

Biological Activity of Vincristine sulfate

Description

Vincristine sulfate is an antitumor vinca alkaloid which inhibits microtubule formation in mitotic spindle, resulting in an arrest of dividing cells at the metaphase stage. It binds to microtubule with a Ki of 85 nM.

In vitro

Delivery of vincristine sulfate-conjugated gold nanoparticles using liposomes: a light-responsive nanocarrier with enhanced antitumor efficiency.[Pubmed: 25960649]

Int J Nanomedicine. 2015 Apr 22;10:3081-95.

The present study explored the use of drug-gold nanoparticle conjugates incorporated into liposomes to enhance antitumor efficiency. A model drug, Vincristine sulfate, was physically conjugated with gold nanoparticles and verified by UV-visible and fourier transform infrared spectroscopy, and differential scanning calorimetry.
METHODS AND RESULTS:
The conjugates were incorporated into liposomes by film dispersion to yield nanoparticles (113.4 nm) with light-responsive release properties, as shown by in vitro release studies. Intracellular uptake and distribution was studied in HeLa cells using transmission electron microscopy and confocal laser scanning microscopy. This demonstrated liposome internalization and localization in endosomal-lysosomal vesicles. Fluorescence intensity increased in cells exposed to UV light, indicating that this stimulated intracellular drug release; this finding was confirmed by quantitative analyses using flow cytometry. Antitumor efficacy was evaluated in HeLa cells, both in culture and in implants in vivo in nude mice.
CONCLUSIONS:
HeLa cell viability assays showed that light exposure enhanced liposome cytotoxicity and induction of apoptosis. Furthermore, treatment with the prepared liposomes coupled with UV light exposure produced greater antitumor effects in nude mice and reduced side effects, as compared with free Vincristine sulfate.

Erythrocyte dysplasia in peripheral blood smears from 5 thrombocytopenic dogs treated with vincristine sulfate.[Pubmed: 24138476]

Vet Clin Pathol. 2013 Dec;42(4):458-64.

Secondary dyserythropoiesis has been associated with vincristine administration in dogs. Evaluation of bone marrow aspirates for the presence of morphologic abnormalities in the erythroid lineage aids in the diagnosis. However, morphologic features of circulating erythroid precursors in these cases have not been described previously. The purpose of this report was to describe the cytologic features of dyserythropoiesis in peripheral blood and also bone marrow smears in a case series of dogs with immune-mediated thrombocytopenia (IMT) treated with Vincristine sulfate.

In vivo

High-dose vincristine sulfate liposome injection (Marqibo) Is not associated with clinically meaningful hematologic toxicity.[Pubmed: 24417913]

Clin Lymphoma Myeloma Leuk. 2014 Jun;14(3):197-202.

Vincristine sulfate liposome injection at its approved dose resulted in a low incidence of clinically meaningful hematologic toxicity. A near doubling of the median dose density did not have an identifiable effect on the reported incidence and severity of hematologic AEs. Vincristine sulfate liposome injection could be well suited for use combined with myelosuppresive drugs and for patients unable to tolerate peripheral blood cytopenia.

Protocol of Vincristine sulfate

Structure Identification

J Drug Target. 2014 Jul;22(6):509-17.

Preparation of vincristine sulfate-loaded poly (butylcyanoacrylate) nanoparticles modified with pluronic F127 and evaluation of their lymphatic tissue targeting.[Pubmed: 24625287]

In order to improve the lymphatic targeting efficiency of anti-cancer agent Vincristine sulfate (VCR), the poly (butylcyanoacrylate) nanoparticles (VCR-PBCA-NPs) were prepared by emulsion polymerization and modified superficially with Pluronic F127.
METHODS AND RESULTS:
These prepared nanoparticles with (F127-VCR-PBCA-NPs) and without surface modification (VCR-PBCA-NPs) were characterized and their lymphatic targeting efficiencies were evaluated in vitro and in vivo. The results showed that Vincristine sulfate was released more sustained from both kinds of Vincristine sulfate-loaded nanoparticles, compared with the Vincristine sulfate solution. The up-taking efficiency of Vincristine sulfate into raji cells was enhanced by F127-VCR-PBCA-NPs, compared with the VCR-PBCA-NPs or Vincristine sulfate solution. Lower clearance (CL) of Vincristine sulfate from the systemic circulation and higher lymphatic targeting efficiency of Vincristine sulfate were observed for F127-VCR-PBCA-NPs than the VCR-PBCA-NPs or Vincristine sulfate solution, and F127-VCR-PBCA-NPs showed greater antitumor efficacy than the VCR-PBCA-NPs or Vincristine sulfate solution in the human Burkitt's lymphoma (raji)-bearing nude mice.
CONCLUSIONS:
These findings suggest that superficially modified nanoscale carriers might be promising vehicles for chemotherapeutic agents in the treatments of metastatic tumors and malignant lymphoma.

Preparing Stock Solutions of Vincristine sulfate

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	1.0834 mL	5.4169 mL	10.8338 mL	21.6675 mL	27.0844 mL
5 mM	0.2167 mL	1.0834 mL	2.1668 mL	4.3335 mL	5.4169 mL
10 mM	0.1083 mL	0.5417 mL	1.0834 mL	2.1668 mL	2.7084 mL
50 mM	0.0217 mL	0.1083 mL	0.2167 mL	0.4334 mL	0.5417 mL
100 mM	0.0108 mL	0.0542 mL	0.1083 mL	0.2167 mL	0.2708 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.