5-Hydroxy-3',4',7-trimethoxyflavone CAS NO.29080-58-8

Min.Order: 10 Milligram
Payment Terms: T/T
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Product Details

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Quick Details

ProName: 5-Hydroxy-3',4',7-trimethoxyflavone
CasNo: 29080-58-8
Molecular Formula: C18H16O6=328.3
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of 5-Hydroxy-3',4',7-trimethoxyflavone

Cas No.	29080-58-8	SDF
PubChem ID	5272653	Appearance	Yellow powder
Formula	C₁₈H₁₆O₆	M.Wt	328.3
Type of Compound	Flavonoids	Storage	Desiccate at -20°C
Solubility	Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name	2-(3,4-dimethoxyphenyl)-5-hydroxy-7-methoxychromen-4-one
SMILES	COC1=C(C=C(C=C1)C2=CC(=O)C3=C(C=C(C=C3O2)OC)O)OC
Standard InChIKey	HIXDQWDOVZUNNA-UHFFFAOYSA-N
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of 5-Hydroxy-3',4',7-trimethoxyflavone

The herbs of Callicarpa bodinieri Levl.

Biological Activity of 5-Hydroxy-3',4',7-trimethoxyflavone

Description

5-Hydroxy-3',4',7-trimethoxyflavone has anti-inflammatory, antibacterial and antifungal activities, it also shows moderate antitrypanosomal activity with MIC value of 19.0 ug/ml. 5-Hydroxy-3',4',7-trimethoxyflavone shows prominent inhibitory activity against soybean lipoxygenase, it can significantly inhibit nitric oxide production and induce reduction in the mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2 in lipopolysaccharide-induced inflammation in RAW 264.7 macrophages.

Targets

LOX | TNF-α | IL Receptor | PGE | COX | NOS | Antifection

In vitro

Protective effect of 5-hydroxy-3′,4′,7-trimethoxyflavone against inflammation induced by lipopolysaccharide in RAW 264.7 macrophage: in vitro study and in silico validation.[Reference: WebLink]

Med. Chem. Res.,2016,25(9):1754-67.

The herb Lippia nodiflora L. (Verbenaceae) has been documented to exhibit anti-inflammatory, antipyretic, antitussive, antidiabetic, anticancer, and antimelanogenesis properties. In the present study, we aimed at evaluating the anti-inflammatory activity of 5-Hydroxy-3',4',7-trimethoxyflavone, a flavonoid from L. nodiflora, using lipopolysaccharide induced inflammation in RAW 264.7 macrophages.
METHODS AND RESULTS:
5-hydroxy-3′,4′,7-trimethoxyflavone significantly inhibited nitric oxide production and demonstrated slight reduction in prostaglandin-E2 level at tested concentrations. The production of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β, were obviously reduced by 5-Hydroxy-3',4',7-trimethoxyflavone in a concentration-dependent manner. Moreover, 5-Hydroxy-3',4',7-trimethoxyflavone significantly induced reduction in the mRNA expressions of inducible nitric oxide synthase and cyclooxygenase-2, representing that inhibition occurs at the transcriptional level. In addition, we performed molecular docking and molecular dynamic simulations to study the interaction of 5-Hydroxy-3',4',7-trimethoxyflavone with inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase-2. Docking study showed its hydrogen bond interactions with key residues in the active site of inducible nitric oxide synthase and cyclooxygenase-2, enlightening its possible binding mode at the molecular level. The results of molecular dynamic simulations showed the stability of complexes and their interactions.
CONCLUSIONS:
Taken together, these findings envisage 5-Hydroxy-3',4',7-trimethoxyflavone as a potential candidate molecule for the progress of therapeutic strategy against inflammation-related diseases.

Antifungal Flavonoids from Ballota glandulosissima.[Reference: WebLink]

Pharm. Biol.,2008, 41(7):483-6.

The flavonoids kumatakenin (1), pachypodol (2), 5-Hydroxy-3',4',7-trimethoxyflavone (3), velutin (4), salvigenin (5), retusin (6) and corymbosin (7) have been isolated from the aerial parts of Ballota glandulosissima Hub.-Mor & Patzak. Among them, 2–4 and 7 have not been reported previously in the genus Ballota.
METHODS AND RESULTS:
The antibacterial and antifungal activities of 1–4 and 6 were tested against Bacillus subtilis, Staphylococcus aureus, Staphylococcus faecalis, Echerichia coli, Pseudomonas aeruginosa, Candida albicans, Candida krusei and Candida galabrata.

Anti-infective Activities of Secondary Metabolites from Vitex pinnata.[Reference: WebLink]

J.Appl. Pharm. Sci.,2016,6(1):102-6.

The phytochemical investigation of Vitex pinnata led to the isolation of a mixture of steroids β-sitosterol and stigmasterol (1a and 1b) and three known flavonoid identified as 5-Hydroxy-3',4',7-trimethoxyflavone(2), 5-hydroxy-7,4’-dimethoxy-flavone (3) and 5-hydroxy-3,3’,4’,7-tetramethoxyflavone (4). The structures of all isolated compounds were carried out by NMR and mass spectrometry.
METHODS AND RESULTS:
The isolated compounds were evaluated for their anti-infective activities against Trypanosoma brucei brucei and Microbacterium marinum. Compound 1-4 showed moderate antitrypanosomal activity with MIC values of 6.25μg/ml, 19.0, 21.0 and 17.0μM, respectively while no activity observed on anti-mycobacterial.
CONCLUSIONS:
This study is the first to report the presence of three flavones and their antitrypanosomal activity from V. pinnata.

Protocol of 5-Hydroxy-3',4',7-trimethoxyflavone

Kinase Assay

Anti-allergenic activity of polymethoxyflavones from Kaempferia parviflora.[Reference: WebLink]

In vitro, fluorescence-quenching and computational studies on the interaction between lipoxygenase and 5-hydroxy-3',4',7-trimethoxyflavone from Lippia nodiflora L.[Pubmed: 26155726]

J Recept Signal Transduct Res. 2015 Jul 9:1-9.

The folk use of L. nodiflora was validated using the isolated natural compound, 5-Hydroxy-3',4',7-trimethoxyflavone (HTMF) by in vitro, fluorescence spectroscopic and molecular modeling studies with lipoxygenase (LOX), because LOX plays an essential role in inflammatory responses.
METHODS AND RESULTS:
In this perspective, the methanol extract and 5-Hydroxy-3',4',7-trimethoxyflavone are shown to demonstrate prominent inhibitory activity against soybean lipoxygenase, with an IC50 value of 21.12 and 23.97 µg/ml, respectively. The data obtained from the spectroscopic method revealed that the quenching of intrinsic fluorescence of LOX is produced as a result of the complex formation of LOX-5-Hydroxy-3',4',7-trimethoxyflavone. The binding mode analysis of 5-Hydroxy-3',4',7-trimethoxyflavone within the LOX enzyme suggested that hydrogen bond formation, hydrophobic interaction and π-π stacking could account for the binding of 5-Hydroxy-3',4',7-trimethoxyflavone. Molecular dynamics results indicated the interaction of 5-Hydroxy-3',4',7-trimethoxyflavone with LOX and the stability of ligand-enzyme complex was maintained throughout the simulation.
CONCLUSIONS:
The computational results are reliable with experimental facts and provided a good representation for understanding the binding mode of 5-Hydroxy-3',4',7-trimethoxyflavone inside the active site of lipoxygenase enzyme.

Journal of Functional Foods, 2015,13:100-7.

The inhibitory effects of 13 polymethoxyflavones (PMFs) obtained from Kaempferia parviflora extracts were evaluated on RBL-2H3 cell degranulation.
METHODS AND RESULTS:
These flavones suppressed antigen-induced degranulation in RBL-2H3 cells. 5-Hydroxy-3',4',7-trimethoxyflavone (KP02) and 5,3′-dihydroxy-3,7,4′,-trimethoxyflavone (KP10) showed potent inhibitory activities. KP10 significantly suppressed calcium ionophores, such as A23187 and 2,5-ditert-butylhydroquinone, which promoted calcium influx from the outside of cells and from the endoplasmic reticulum, respectively. However, these activities did not fully explain the PMFs' effects. Immunoblot analysis of KP02 and KP10 showed that the inhibitory effects were attributable to the downregulation of PLCγ1 kinase phosphorylation in the signaling pathways involved in degranulation. The phosphorylation levels of Syk were also suppressed by the addition of KP10. KP02 and KP10 treatment to the cells did not affect the high affinity IgE receptor (FcεRI) levels inside RBL-2H3 cells, but they suppressed the FcεRI levels in the plasma membrane.
CONCLUSIONS:
Therefore, K. parviflora PMFs have multiple degranulation mechanisms to suppress RBL-2H3 cells and would be beneficial for alleviating type I allergy symptoms.

Preparing Stock Solutions of 5-Hydroxy-3',4',7-trimethoxyflavone

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	3.046 mL	15.23 mL	30.4599 mL	60.9199 mL	76.1499 mL
5 mM	0.6092 mL	3.046 mL	6.092 mL	12.184 mL	15.23 mL
10 mM	0.3046 mL	1.523 mL	3.046 mL	6.092 mL	7.615 mL
50 mM	0.0609 mL	0.3046 mL	0.6092 mL	1.2184 mL	1.523 mL
100 mM	0.0305 mL	0.1523 mL	0.3046 mL	0.6092 mL	0.7615 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.