Abietin CAS NO.531-29-3

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Product Details

Keywords

Abietin
531-29-3
standard supplier in China

Quick Details

ProName: Abietin
CasNo: 531-29-3
Molecular Formula: C16H22O8=342.3
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Coniferin

Cas No.	531-29-3	SDF
PubChem ID	5280372	Appearance	Powder
Formula	C₁₆H₂₂O₈	M.Wt	342.3
Type of Compound	Phenylpropanoids	Storage	Desiccate at -20°C
Synonyms	(E)-Coniferin;124151-33-3;Coniferyl alcohol beta-D-glucoside;Abietin
Solubility	Soluble in Chloroform,Dichloromethane,Ethyl Acetate,DMSO,Acetone,etc.
Chemical Name	(2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-[4-[(E)-3-hydroxyprop-1-enyl]-2-methoxyphenoxy]oxane-3,4,5-triol
SMILES	COC1=C(C=CC(=C1)C=CCO)OC2C(C(C(C(O2)CO)O)O)O
Standard InChIKey	SFLMUHDGSQZDOW-FAOXUISGSA-N
Standard InChI	InChI=1S/C16H22O8/c1-22-11-7-9(3-2-6-17)4-5-10(11)23-16-15(21)14(20)13(19)12(8-18)24-16/h2-5,7,12-21H,6,8H2,1H3/b3-2+/t12-,13-,14+,15-,16-/m1/s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Coniferin

The herbs of Acanthopanax gracilistylus

Biological Activity of Coniferin

Description

Coniferin has ATP-dependent transport activity; the extracts of the Balanophora involucrate and trans-Coniferin have anti-oxidation effects; the chromogenic coniferin analog show the exclusive presence of beta-glucosidase activity in the differentiating xylem, similar to peroxidase activity.

Targets

ATP | beta-Glucosidase

In vitro

A beta-glucosidase from lodgepole pine xylem specific for the lignin precursor coniferin.[Pubmed: 7724669]

Plant Physiol. 1995 Feb;107(2):331-9.

Coniferin, the glucoside of the monolignol coniferyl alcohol, accumulates to high levels in gymnosperms during spring-cambial reactivation. A cinnamyl alcohol glucoside/beta-glucosidase system is thought to play a key role in lignification by releasing the monolignol aglycones. Investigation of such an enzyme system in the xylem of Pinus contorta var latifolia Engelm. revealed two major beta-glucosidases.
METHODS AND RESULTS:
One efficiently hydrolyzed the native substrate, Coniferin, and the other was more active against synthetic glucosides. The Coniferin beta-glucosidase was purified to apparent homogeneity using anion exchange, hydrophobic interaction, and size-exclusion chromatography. The apparent native molecular weight was estimated to be 60,000. A dominant 28-kD protein and a minor 24-kD protein were detected in the purified preparation following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunological evidence from polyclonal antibodies directed against the synthetic N-terminal peptide of the 24-kD protein suggested that the native protein is a dimer of 28-kD subunit size.
CONCLUSIONS:
The N-terminal sequence showed that Coniferin beta-glucosidase has high homology to known plant beta-glucosidases. Coniferin, syringin, and a synthetic Coniferin analog were preferred substrates for the Coniferin beta-glucosidase. In situ localization using the chromogenic Coniferin analog showed the exclusive presence of beta-glucosidase activity in the differentiating xylem, similar to peroxidase activity.

Study of Anti-oxidation Effects of Extracts of Balanophora Involucrate and Trans-Coniferin.[Reference: WebLink]

Journal of China Three Gorges University, 2009, 31(3):99-101.

METHODS AND RESULTS:
To study the anti-oxidation effect of extracts of Balanophora involucrate and trans-Coniferin,using the methods of DHHP to study the anti-oxidation effect of extracts of Balanophora involucrate and trans-Coniferin.When the concentration of the extracts of Balanophora involucrate is 3 mg/mL,the absorbency is the least;other concentrations can definitly eliminate the DPPH free radical.
CONCLUSIONS:
The conclusion is that both the extracts of the Balanophora involucrate and trans-Coniferin have anti-oxidation effects.

Protocol of Coniferin

Kinase Assay

Proton-dependent coniferin transport, a common major transport event in differentiating xylem tissue of woody plants.[Pubmed: 23585651]

Plant Physiol. 2013 Jun;162(2):918-26.

Lignin biosynthesis is an essential physiological activity of vascular plants if they are to survive under various environmental stresses on land. The biosynthesis of lignin proceeds in the cell wall by polymerization of precursors; the initial step of lignin polymerization is the transportation of lignin monomers from the cytosol to the cell wall, which is critical for lignin formation. There has been much debate on the transported form of the lignin precursor, either as free monolignols or their glucosides.
METHODS AND RESULTS:
In this study, we performed biochemical analyses to characterize the membrane transport mechanism of lignin precursors using angiosperms, hybrid poplar (Populus sieboldii × Populus grandidentata) and poplar (Populus sieboldii), as well gymnosperms, Japanese cypress (Chamaecyparis obtusa) and pine (Pinus densiflora). Membrane vesicles prepared from differentiating xylem tissues showed clear ATP-dependent transport activity of Coniferin, whereas less than 4% of the Coniferin transport activity was seen for coniferyl alcohol. Bafilomycin A1 and proton gradient erasers markedly inhibited Coniferin transport in hybrid poplar membrane vesicles; in contrast, vanadate had no effect. Cis-inhibition experiments suggested that this transport activity was specific for Coniferin. Membrane fractionation of hybrid poplar microsomes demonstrated that transport activity was localized to the tonoplast- and endomembrane-rich fraction.
CONCLUSIONS:
Differentiating xylem of Japanese cypress exhibited almost identical transport properties, suggesting the involvement of a common endomembrane-associated proton/Coniferin antiport mechanism in the lignifying tissues of woody plants, both angiosperms and gymnosperms.

Preparing Stock Solutions of Coniferin

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.9214 mL	14.6071 mL	29.2141 mL	58.4283 mL	73.0353 mL
5 mM	0.5843 mL	2.9214 mL	5.8428 mL	11.6857 mL	14.6071 mL
10 mM	0.2921 mL	1.4607 mL	2.9214 mL	5.8428 mL	7.3035 mL
50 mM	0.0584 mL	0.2921 mL	0.5843 mL	1.1686 mL	1.4607 mL
100 mM	0.0292 mL	0.1461 mL	0.2921 mL	0.5843 mL	0.7304 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.