Schizandrin B CAS NO.61281-37-6

Min.Order: 10 Milligram
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Schizandrin B
61281-37-6
standard supplier in China

Quick Details

ProName: Schizandrin B
CasNo: 61281-37-6
Molecular Formula: C23H28O6=400.47
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Schizandrin B

Cas No.	61281-37-6	SDF
PubChem ID	108130	Appearance	White powder
Formula	C₂₃H₂₈O₆	M.Wt	400.47
Type of Compound	Lignans	Storage	Desiccate at -20°C
Synonyms	Schizandrin-B; Wuweizisu-B; gamma-Schisandrin
Solubility	DMSO : 14.29 mg/mL (35.68 mM; Need ultrasonic) H2O : < 0.1 mg/mL (insoluble)
SMILES	CC1CC2=CC3=C(C(=C2C4=C(C(=C(C=C4CC1C)OC)OC)OC)OC)OCO3
Standard InChIKey	RTZKSTLPRTWFEV-UHFFFAOYSA-N
Standard InChI	InChI=1S/C23H28O6/c1-12-7-14-9-16(24-3)20(25-4)22(26-5)18(14)19-15(8-13(12)2)10-17-21(23(19)27-6)29-11-28-17/h9-10,12-13H,7-8,11H2,1-6H3
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Schizandrin B

The seeds of Schisandra chinensis (Turcz.) Baill.

Biological Activity of Schizandrin B

Description	Schisandrin B, a kind of ATR and P-gp inhibitor with high safety, has been shown to produce antioxidant effect on rodent liver and heart. It also has anti-photoaging, and presents promising activities for future development of protective agents against CisPt nephrotoxicity. Combination of schizandrin B and paclitaxel(PTX) can enhance anti-tumor effects and relieve side effects of PTX on rats with mammary carcinoma.
Targets	Wnt/β-catenin \| Caspase \| p53 \| P21 \| P450 (e.g. CYP17) \| P-gp \| Fas \| ATR
In vitro	Protective effects of schizandrin and schizandrin B towards cisplatin nephrotoxicity in vitro.[Pubmed: 24155209] J Appl Toxicol. 2014 Dec;34(12):1311-9. Renal proximal tubular epithelial cells are the main targets of toxic drugs such as cisplatin (CisPt), an alkylating agent indicated for the treatment of solid organ tumors. Current techniques aiming at reducing nephrotoxicity in patients receiving CisPt are still not satisfactory as they can only partially prevent acute kidney injury. New nephroprotective strategies remain to be developed. METHODS AND RESULTS: In the present in vitro study, schizandrin (Schi) and Schizandrin B (Schi B), major phytochemicals from Schisandra chinensis (Turcz.) Baill. fruits, were tested on HK-2 cells along four processes that could help alleviate CisPt toxicity. Results indicated that: (i) both Schi and Schi B enhanced cell survival via reducing apoptosis rate; (ii) only Schi showed moderate effects towards modulation of regeneration capacities of healthy cells; (iii) both Schi and Schi B limited extracellular matrix deposition; and (iv) both compounds could help preventing dedifferentiation processes via the β-catenin pathway. CONCLUSIONS: Schi and Schi B present promising activities for future development of protective agents against CisPt nephrotoxicity. Action of schizandrin B, an antioxidant, on lipid peroxidation in primary cultured hepatocytes.[Pubmed: 2624122] Zhongguo Yao Li Xue Bao. 1989 Jul;10(4):353-6. METHODS AND RESULTS: The action of Schizandrin B (Sin B) was observed in freshly isolated hepatocytes damaged by FeSO4/cysteine and CCl4. Two types of free radicals, .OH and .CCl3, generated from FeSO4/cysteine and CCl4, respectively, induced lipid peroxidation in hepatocytes. It was found that the speed of lipid peroxidation (MDA production) and the degree of alteration in hepatocyte morphology were closely related to the type of free radicals. MDA production and membrane protrusion of hepatocytes injuries by FeSO4/cysteine were faster and more severe than those observed with CCl4. Sin B was shown to decrease the production of MDA and the release of GPT and LDH, and to increase hepatocyte viability as well as maintaining the integrity of the hepatocyte membrane surface. These actions of Sin B were stronger than vitamin E at the same concentration. It was observed that no inhibitory effect of phenobarbital, a typical inducer of cytochrome P-450, as Sin B induced liver cytochrome P-450, on MDA production in hepatocytes damaged by FeSO4/cysteine. CONCLUSIONS: The results suggest that Sin B possesses antioxidant activity.
In vivo	Experimental research on Schizandrin B enhancing sensitivity to paclitaxel on rats with mammary carcinoma.[Reference: WebLink] J. Pract. Med., 2012, 28(15):2506-9. To investigate the effects of Schizandrin B (Sch B) on enhancing sensitivity to paclitaxel (PTX) on rats with mammary carcinoma. METHODS AND RESULTS: Fifty BALB/c rats were randomly divided into five groups, 10 animals of each group: a normal control (NC) group, model control (MC) group, PTX group, Sch B group and PTX + Sch B group. Sch B was administered to the rats by intraperitoneal injection at a dose of 10mg/kg once per day for 22 days. while PTX was every 2 days. The body weight, gross tumor volume, inhibition rate of tumor growth and organ index were observed. Compared with the MC group, the body weight in both the PTX group and Sch B + PTX group began to decrease since the 7th day of administration, the difference in weight was significant by statistics. Compared with the PTX group, the body weight in the Sch B + PTX group was elevated, but the difference was not significant. The inhibition rate in the PTX group was 15.7% and that in the Sch B + PTX group 25.9%. Compared with the MC group, the spleen index in PTX group and the Sch B + PTX group was significantly lower (P = 0.008 and 0.000, respectively). Compared with the PTX group, the spleen index in the Sch B + PTX group was lower, but the difference was not significant. Compared with the PTX group, the thyroid and adrenal gland index in the Sch B + PTX group was significantly elevated (P = 0.000 and 0.011, respectively). CONCLUSIONS: Combination of Sch B and PTX can enhance anti-tumor effects and relieve side effects of PTX on rats with mammary carcinoma.

Protocol of Schizandrin B

Kinase Assay

[Effect of schizandrin B on H(2)O(2)-induced apoptosis of human hepatocytes in vitro: role of Fas pathway].[Pubmed: 22543149]

Nan Fang Yi Ke Da Xue Xue Bao. 2012 Apr;32(4):583-5, 592.

To investigate the role of Fas pathway in H(2)O(2)-induced apoptosis of L02 human hepatocytes and the effect of schisandrin B on Fas pathway.
METHODS AND RESULTS:
Real-time quantitative PCR was used to detect the expressions of FAS, fas associated death domain protein (FADD) and caspase-8 mRNA in L02 cells exposed to H(2)O(2). Flow cytometry was employed to assess the cell apoptosis. ELISA, Western blotting and spectrophotometric assay were performed to determine the expressions of FAS protein, FADD protein and caspase-8 activity. Within the dose range of 5-15 mol/L, schisandrin B dose-dependently inhibited FAS and FADD expressions and caspase-8 activation.
CONCLUSIONS:
Schisandrin B can partially inhibit H(2)O(2)-induced L02 cell apoptosis possibly by affecting the FAS-FADD-caspase-8 pathway.

Cell Research

Protective effect of schizandrin B against oxidative damage of UVB irradiated HaCaT cells and its molecular mechanism.[Reference: WebLink]

Chinese Pharmacological Bulletin, 2014, 30(4):523-7.

To investigate the inhibitory effect of Schizandrin B (SchB) on ultraviolet radiation b (UVB) radiation-induced apoptosis of HaCaT cells.
METHODS AND RESULTS:
Methyl thiazolyl tetrazolium (MTT) assay was used to examine the effect of SchB on cell viability recovery. Cell apoptosis and necrosis were measured by Hochest33342 staining. The p53, p21 and Caspase-3 mRNA expressions were examined by RT-PCR. In this study, we found that Sch B attenuated UVB-induced toxicity in HaCaT cells. Through Hoechst 33342 stain, we visualized that SchB could inhibit UVB-induced HaCaT cell death. The result demonstrated that p53, p21 and Caspase-3 mRNA levels decreased compared with the control group.
CONCLUSIONS:
Sch B attenuates the UVB-induced toxicity of HaCaT by inhibiting apoptotic gene expression. It plays a role in antiphotoaging.

Preparing Stock Solutions of Schizandrin B

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.4971 mL	12.4853 mL	24.9707 mL	49.9413 mL	62.4266 mL
5 mM	0.4994 mL	2.4971 mL	4.9941 mL	9.9883 mL	12.4853 mL
10 mM	0.2497 mL	1.2485 mL	2.4971 mL	4.9941 mL	6.2427 mL
50 mM	0.0499 mL	0.2497 mL	0.4994 mL	0.9988 mL	1.2485 mL
100 mM	0.025 mL	0.1249 mL	0.2497 mL	0.4994 mL	0.6243 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.