Corynoxine CAS NO.6877-32-3

Min.Order: 10 Milligram
Payment Terms: T/T
Available Specifications:

Product Details

Keywords

Corynoxine
6877-32-3
standard supplier in China

Quick Details

ProName: Corynoxine
CasNo: 6877-32-3
Molecular Formula: C22H28N2O4
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Corynoxine

Cas No.	6877-32-3	SDF
PubChem ID	10475115	Appearance	Cryst.
Formula	C₂₂H₂₈N₂O₄	M.Wt	384.47
Type of Compound	Alkaloids	Storage	Desiccate at -20°C
Solubility	DMSO : ≥ 100 mg/mL (260.10 mM) H2O : < 0.1 mg/mL (insoluble) *"≥" means soluble, but saturation unknown.
Chemical Name	methyl (E)-2-[(3S,6'S,7'S,8'aS)-6'-ethyl-2-oxospiro[1H-indole-3,1'-3,5,6,7,8,8a-hexahydro-2H-indolizine]-7'-yl]-3-methoxyprop-2-enoate
SMILES	CCC1CN2CCC3(C2CC1C(=COC)C(=O)OC)C4=CC=CC=C4NC3=O
Standard InChIKey	DAXYUDFNWXHGBE-NRAMRBJXSA-N
Standard InChI	InChI=1S/C22H28N2O4/c1-4-14-12-24-10-9-22(17-7-5-6-8-18(17)23-21(22)26)19(24)11-15(14)16(13-27-2)20(25)28-3/h5-8,13-15,19H,4,9-12H2,1-3H3,(H,23,26)/b16-13+/t14-,15+,19+,22+/m1/s1
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Corynoxine

The vine of Uncaria rhynchopylla

Biological Activity of Corynoxine

Description	Corynoxine is a natural autophagy enhancer, promotes the clearance of Alpha-synuclein via Akt/mTOR pathway, it can significantly inhibit the oxidative stress of PC12 cells.
Targets	Akt \| mTOR \| Autophagy
In vitro	Study on application of statistical modeling on chromatogram-effect relation in Traditional Chinese Medicine[Reference: WebLink] Internal Medicine of China,2013,8(6):586-7. To establish chromatogram-effect model based on inhibitory effect of four alkaloids( rhynchophyline,isorhynchophylline,Corynoxine,Corynoxine B) on oxidative stress of glutamate-induced PC12 cells. METHODS AND RESULTS: Based on the chromatogram-effect relation of inhibitory effect of four alkaloids on the oxidative stress of glutamate-induced PC12 cells,SPSS 15. 0 was used to analyse data. Oxidative stress of PC12 cells was significantly inhibited by rhynchophyline and Corynoxine. The spectrum efficient equation was,Y = 44. 301 + 0. 255X 1 + 0. 356X 3( P 0. 05). The deviation of predictive value and true value was no more than 10% by data validation from seven samples. CONCLUSIONS: The chromatogram-effect model can be used to predict the effect of uncaria macrophylla wall against the oxidative stress of glutamate-induced PC12 cells.
In vivo	Effect on locomotion of indole alkaloids from the hooks of uncaria plants.[Pubmed: 10439480] Phytomedicine. 1999 Jul;6(3):163-8. METHODS AND RESULTS: Three predominant Uncariae plants, Uncaria rhynchophylla U. sinensis and U. macrophylla and their indole and oxindole alkaloid constituents were studied for their effect on locomotor response. Water extracts of U. macrophylla and U. sinensis and four indole alkaloids, Corynoxine, Corynoxine B, isorhynchophylline and geissoschizine methyl ether, significantly decreased locomotor activity after oral administration to mice. CONCLUSIONS: The depression of locomotor activity upon administration of these alkaloids appears to be due to mediating of the central dopaminergic system.

Protocol of Corynoxine

Kinase Assay

Corynoxine, a natural autophagy enhancer, promotes the clearance of alpha-synuclein via Akt/mTOR pathway.[Pubmed: 24522518]

J Neuroimmune Pharmacol. 2014 Jun;9(3):380-7.

Parkinson's disease (PD) is the second most common neurodegenerative disorder characterized by the accumulation of protein aggregates (namely Lewy bodies) in dopaminergic neurons in the substantia nigra region of the brain. Alpha-synuclein (α-syn) is the major component of Lewy bodies in PD patients, and impairment of the autophagy-lysosomal system has been linked to its accumulation. In our previous study, we identified an oxindole alkaloid Corynoxine B (Cory B), isolated from Uncaria rhynchophylla (Miq.) Jacks (Gouteng in Chinese), as a Beclin-1-dependent autophagy inducer.
METHODS AND RESULTS:
In this work, we show that Cory, an enantiomer of Cory B, also induces autophagy in different neuronal cell lines, including N2a and SHSY-5Y cells, which is paralleled with increased lysosomal enzyme cathepsin D. In vivo, Cory promotes the formation of autophagosomes in the fat bodies of Drosophila. By inducing autophagy, Cory promotes the clearance of wild-type and A53T α-syn in inducible PC12 cells. Interestingly, different from its enantiomer Cory B, Cory induces autophagy through the Akt/mTOR pathway as evidenced by the reduction in the levels of phospho-Akt, phospho-mTOR and phospho-p70 S6 Kinase.
CONCLUSIONS:
Collectively, our findings provide experimental evidence for developing Cory as a new autophagy enhancer from Chinese herbal medicine, which may have potential application in the prevention or treatment of PD.

Preparing Stock Solutions of Corynoxine

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.601 mL	13.0049 mL	26.0098 mL	52.0197 mL	65.0246 mL
5 mM	0.5202 mL	2.601 mL	5.202 mL	10.4039 mL	13.0049 mL
10 mM	0.2601 mL	1.3005 mL	2.601 mL	5.202 mL	6.5025 mL
50 mM	0.052 mL	0.2601 mL	0.5202 mL	1.0404 mL	1.3005 mL
100 mM	0.026 mL	0.13 mL	0.2601 mL	0.5202 mL	0.6502 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.