Isoscopoletin CAS NO.776-86-3

Min.Order: 10 Milligram
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ProName: Isoscopoletin
CasNo: 776-86-3
Molecular Formula: C10H8O4=192.17
Appearance: detailed see specifications
Application: analysis,activity test,Botanical Refer...
DeliveryTime: 1-3?working?days?after?confirming?the?...
PackAge: According to the clients requirement.
Port: China main port
ProductionCapacity: 1 Metric Ton/Day
Purity: ≥98%
Storage: Store at 2~8°C
Transportation: by air or by ocean shipping
LimitNum: 10 Milligram
Plant of Origin: Chinese herbal medicine
Testing Method: NMR/MS/HPLC
Product Ecification: 1mg-1kg
Heavy Metal: <10ppm
Voluntary Standards: company standard
Storage: Store in dry, dark and ventilated plac...
PackAge: Brown vial HDPE plastic bottle

Superiority

Hubei CuiRan Biotechnology Co., Ltd is a leading company in the research, development, manufacture and marketing of High Quality Phytochemicals and Extracts(especially Active Ingredients from Traditional Chinese Medicine，Traditional Chinese Medicine), Natural Active Pharmaceutical Ingredients worldwide. From small quantities for R&D or reference standard, to large quantities for customizing or manufacturing, Biopurify emphasizes on consistent and reliable services for his customers.
With excellent quality products and good service, we have clients from more than dozens countries and regions, and we pride ourselves in providing our customers with a total satisfaction experiences.
We are doing our best to be your reliable partner for high quality Phytochemicals and Reference Standards from china.

Our main services:
A. Supply active ingredients and reference standards ofTraditional Chinese Medicine, from mgs to kgs scale.
B. Custom extraction and purification, target Herb Active Ingredients
C. Custom synthesis and semi-synthesis for Natural Active Ingredients
D. CR, CM and PD services from lab scale, pilot scale to commercial scale(GMP is also available)
E.Traditional Chinese Medicine compounds library

1.Provide traditional Chinese medicine reference materials and natural active ingredients;
2.More than 2200 compounds are available for selection, continuously building high-quality natural product libraries for drug research and development;
3.Provide various screening libraries and more inhibitor products;
4.Provide separation and structural determination of natural products;
5.Laboratory scale pilot to commercial scale collaborative research and process development services.More than 180 experiences in phytochemistry (still increasing)
Each product has passed very strict testing (NMR/MS/HPLC)
Agents from many countries

General tips：For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months.
Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging：1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial.
2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial.
3. Try to avoid loss or contamination during the experiment.
Shipping Condition：Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Details

Chemical Properties of Isoscopoletin

Cas No.	776-86-3	SDF
PubChem ID	69894	Appearance	White-beige powder
Formula	C₁₀H₈O₄	M.Wt	192.17
Type of Compound	Coumarins	Storage	Desiccate at -20°C
Synonyms	Esculetin 7-methyl ether; 6-Hydroxy 7-methoxycoumarin; 7-Methoxyesculetin
Solubility	Soluble in chloroform and pyridine
Chemical Name	6-hydroxy-7-methoxychromen-2-one
SMILES	COC1=C(C=C2C=CC(=O)OC2=C1)O
Standard InChIKey	SYTYLPHCLSSCOJ-UHFFFAOYSA-N
Standard InChI	InChI=1S/C10H8O4/c1-13-9-5-8-6(4-7(9)11)2-3-10(12)14-8/h2-5,11H,1H3
General tips	For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. We recommend that you prepare and use the solution on the same day. However, if the test schedule requires, the stock solutions can be prepared in advance, and the stock solution must be sealed and stored below -20℃. In general, the stock solution can be kept for several months. Before use, we recommend that you leave the vial at room temperature for at least an hour before opening it.
About Packaging	1. The packaging of the product may be reversed during transportation, cause the high purity compounds to adhere to the neck or cap of the vial.Take the vail out of its packaging and shake gently until the compounds fall to the bottom of the vial. 2. For liquid products, please centrifuge at 500xg to gather the liquid to the bottom of the vial. 3. Try to avoid loss or contamination during the experiment.
Shipping Condition	Packaging according to customer requirements(5mg, 10mg, 20mg and more). Ship via FedEx, DHL, UPS, EMS or other couriers with RT, or blue ice upon request.

Source of Isoscopoletin

1 Euphorbia sp. 2 Fraxinus sp. 3 Mentzelia sp. 4 Morinda sp. 5 Olea sp. 6 Saussurea sp.

Biological Activity of Isoscopoletin

Description

Isoscopoletin possesses inhibitory activity against hepatitis B virus (HBV) replication; it also shows substantial inhibition against multi-drug resistant CEM/ADR5000 cells and human CCRF-CEM leukaemia cells, with the IC50 value is 1.6 and 4.0 microM, respectively.

Targets

HBV

In vitro

Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi.[Pubmed: 16881019]

Planta Med. 2006 Jul;72(9):862-4.

METHODS AND RESULTS:
The ethyl acetate extract of Artemisia argyi leaves showed substantial inhibition in a cell proliferation assay using human CCRF-CEM leukaemia cells. Bioassay-guided fractionation of the extract led to the isolation of scopoletin and Isoscopoletin as the active principles. Their IC50 values were 2.6 and 4.0 microM, respectively. Additionally the two substances were tested against the multidrug resistant subline, CEM/ADR5000 where they both showed IC50 values of 1.6 microM.
CONCLUSIONS:
In contrast to the standard cytostatic drugs doxorubicin, vincristine, and paclitaxel, CEM/ADR5000 cells therefore did not exhibit cross-resistance to scopoletin and Isoscopoletin.

Evaluation of antiviral activity of compounds isolated from Ranunculus sieboldii and Ranunculus sceleratus.[Pubmed: 16395649 ]

Planta Med. 2005 Dec;71(12):1128-33.

METHODS AND RESULTS:
Nineteen compounds isolated from Ranunculus sieboldii and Ranunculus sceleratus were tested for inhibitory effects on hepatitis B virus (HBV) and Herpes simplex virus type-1 (HSV-1). The results showed that apigenin 4'- O- alpha-rhamnopyranoside, apigenin 7- O- beta-glucopyranosyl-4'- O- alpha-rhamnopyranoside, tricin 7- O- beta-glucopyranoside, tricin, and Isoscopoletin possessed inhibitory activity against HBV replication. Protocatechuyl aldehyde exhibited an inhibiting activity on HSV-1 replication.
CONCLUSIONS:
It is therefore suggested that further investigations on these bioactive compounds might be needed to discover and develop new antiviral agents.

Protocol of Isoscopoletin

Cell Research

Machilusmarin, a new neuroprotective isocoumarin dimer from the stems of Machilus ichangensis Rehd. et Wils.[Pubmed: 23082968 ]

Nat Prod Res. 2013;27(17):1542-7.

A new isocoumarin dimer, Machilusmarin (1), was isolated from the stem of Machilus ichangensis Rehd. et Wils., together with two known coumarins, scopoletin (2) and Isoscopoletin (3).
METHODS AND RESULTS:
The structure of Machilusmarin (1) was elucidated by HRMS and NMR spectrums. Machilusmarin (1) showed significant neuroprotective activity against SH-SY5Y human neuroblastoma cells apoptosis induced by hydrogen peroxide at concentrations ranging from 0.625 to 10.0 μg mL(-1).

Preparing Stock Solutions of Isoscopoletin

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	5.2037 mL	26.0186 mL	52.0373 mL	104.0745 mL	130.0931 mL
5 mM	1.0407 mL	5.2037 mL	10.4075 mL	20.8149 mL	26.0186 mL
10 mM	0.5204 mL	2.6019 mL	5.2037 mL	10.4075 mL	13.0093 mL
50 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.0815 mL	2.6019 mL
100 mM	0.052 mL	0.2602 mL	0.5204 mL	1.0407 mL	1.3009 mL
* Note: If you are in the process of experiment, it's necessary to make the dilution ratios of the samples. The dilution data above is only for reference. Normally, it's can get a better solubility within lower of Concentrations.